The Wallaroos have confirmed they will play Ireland for the first time in seven years at Kingspan Stadium in Belfast, Northern Ireland, on September 14.
Jo Yapp's side will take on the Irish ahead of WXV, with Ireland locking themselves in the top competition after wins over Scotland and Wales.
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The two teams last played at the 2017 Rugby World Cup, with Australia taking a 36-24 win.
Trilleen Pomare is the lone remaining player in the Wallaroos squad from that match, gaining revenge after their defeat in the group stages.
"We are really excited by the opportunity to play Ireland in Belfast ahead of the WXV," Yapp said in a statement.
“Ireland have moved up in the rankings this year and will provide our players with a great challenge of playing against a tough and improved side overseas."
The confirmation of the Test comes as part of an expanded calendar for the Wallaroos in 2024, who entered camp today in Sydney.
They will open their campaign against Canada on May 11 at Allianz Stadium, beginning their Pacific Four Series.
The Australians will also play internationals against Fijiana and New Zealand in July, with a further Test to be confirmed as the build continues for the 2025 Rugby World Cup.
"It's crucial our team is exposed to Test matches like these and away from home leading into the WXV competition," Yapp added.
“We look forward to that match-up when it comes, but for the time being our focus is on starting well against Canada in the Pacific Four series.”